- 2012-2013 Principal Scientist, Bioprocess Development Group, Pfizer, Kalamazoo, MI
- 2010-2012 Postdoctoral Fellow, California Institute of Technology, Pasadena, CA
- 2004-2010 Ph.D. in Medicinal Chemistry, University of Michigan, Ann Arbor, MI
- 2001-2004 M.S. Chemistry, University of Nebraska, Lincoln, NE
- 1996-2000 B.S. Applied Chemistry, Peking University, China
Yousong Ding, Ph.D., an associate professor of medicinal chemistry, received his B.S. in applied chemistry from Peking University. He pursued his M.S. studies about fungal secondary metabolite biosynthesis at the University of Nebraska under the direction of Dr. Liangcheng Du. He further expanded his expertise in the natural product field during his Ph.D. training with Dr. David Sherman at the University of Michigan. Dr. Ding then worked as a postdoctoral scholar in Dr. Frances H. Arnold’s laboratory at the California Institute of Technology. In the Arnold laboratory, he applied principles of protein engineering and synthetic biology to develop biocatalysts for the production of valuable chemicals and to understand herbicide metabolism. His long-term interests in developing drug molecules led him to take a position in Pfizer. This position allowed him to gain experience in pharmaceutical bioprocess development, and correspondingly he generated various types of biocatalysts used in bio-routes for lowering manufacturing costs of several drugs. In 2013, Dr. Ding started his position as an assistant professor in the Department of Medicinal Chemistry at the University of Florida. His research interests include natural product biosynthesis, drug discovery and development, synthetic biology, protein engineering, and chemical biology.
One primary goal of the Ding laboratory is to discover and develop small molecules and biologics as new therapeutic leads that address significant unmet medical needs such as drugs for obesity, cardiovascular, cancer, and infectious diseases. Nature offers a vast array of small molecules that have inspired the development of a number of marketed drugs, and continuously serve as a prolific source of new drug leads. The Ding laboratory is particularly interested in deciphering and employing the biosynthetic logics of these small molecules for drug discovery. One strategy employed in the laboratory involves identification and analysis of natural product biosynthetic systems from microbial genome data, heterologous production of encoded chemicals, structure determination, and bioactivity evaluation. Through designing novel production routes, developing capable hosts, and creating new/improved biocatalysts, the Ding laboratory will optimize chemical productivity and diversify structures of select candidates. In another research area, the Ding laboratory is interested in developing biocatalysts from different types of enzymes in natural products biosynthetic pathways and employing them to produce value-added chemicals. Researchers in the Ding laboratory gain broad training spanning organic chemistry, medicinal chemistry, biochemistry, microbiology, molecular biology, cell biology, protein engineering, synthetic biology, and bioprocess development.
- Peilan Zhang (B.S. in Pharmacy, Sichuan University)
- Manyun Chen (M.S. in Pharmaceutical Science, University of Southern California)
- Dake Liu (B.S. in Medicinal Chemistry, China Pharmaceutical University)
- Garret M. Rubin (B.S. in Biochemistry, University of Michigan)
- Sarah Hylton (undergraduate)
- Michael Guo (undergraduate)
- Zacharry Raad (undergraduate)
- Malhar Patel (undergraduate, Emerging Scholar)
- Leeann Latford (undergraduate)
- Alivia Ishee (undergraduate)
High School Students:
- Julius Chai
- Padmavathi Reddy
- Kathryn Wulber
- Ryan Chen
- Gahyun Ally Lee
- Vincent Huang
- Sunny Aroda
- Harrison J Bonilla
- Erica Christenson
- Monica Cozad
- Steven Crichton
- Evelina Dedic
- Nilay S Dharma
- Wesley Dickerson
- Nicholas Lee
- Kimberly Loudermilk
- Nina Jovic
- Mishal P Mehta
- Michael Patterson
- Magan Powell
- Albert Tieu
- Kyle Volland
- Ashley Wome
- Sara Kearny (Wheaton College)
- Jada Brooks (Bethune-Cookman University)
- Ariana E. Santiago (REU, University of Puerto Rico)
- Destin Holland
- Olufunmilayo Daudu (Florida A&M U)
- Caitlin Cain (REU, VCU)
- Chris Rakowski (REU, Bucknell College)
Pharm. D. Students:
- XiaoBin Chen
- Julian Rashid
- Joshua L McBride
- Jaehyeok Roh
- Deniella Chin-Quee
- Nicholas Hiller
- Stephen Fotopoulos.
- Hongfen Yang
- Yu Shan
- Chao Jiang
- Tariq Ismail
- Stefan Schug
- Ran Zuo (May 2018, Postdoc at UF)
- Guangde Jiang (Aug, 2019, Postdoc at UIUC)
- Yi Zhang (Aug, 2019, Postdoc at Princeton)
- Guang Yang (2014-2017)
University of Florida Affiliations
- Center for Natural Products, Drug Discovery and Development (CNPD3)
- Emerging Pathogens Institute (EPI)
- UF Health Cancer Center
- UF Informatics Institute
- UF Biodiversity Institute
- The National High Magnetic Field Laboratory (NHMFL)
Ding Lab Publications
- Li H, Zhang Y, Wang F, Donelan W, Zona MC, Li S, Reeves W, Ding Y, Tang D, Yang L. Effects of irisin on the differentiation and browning of human visceral white adipocytes. Am. J. Transl. Res. 2019, 11: 7410-7421.
- Zuo R, Ding Y. Direct aromatic nitration system for synthesis of nitrotryptophans in Escherichia coli. ACS Syn. Biol. 2019, 19, 8: 857-865.
- Abouelhassan Y, Zhang P, Ding Y, Huigens RW. Rapid kill assessment of an N-arylated NH125 analogue against drug-resistant microorganisms. MedChemComm 2019, 10: 712-716.
- Gunasekera SP, Meyer JL, Ding Y, Abboud KA, Luo D, Campbell JE, Angerhofer A, Goodsell JL, Raymundo LJ, Liu J, Ye T, Luesch H, Teplitski M, Paul VJ. Chemical and metagenomic studies of the lethal black band disease of corals reveal two broadly distributed, redox-sensitive mixed polyketide/peptide macrocycles. J. Nat. Prod. 2019, 82: 111-121.
- Jiang G, Zhang Y, Powell MM, Hylton SM, Hiller NW, Loria R, Ding Y. A promiscuous cytochrome P450 hydroxylates aliphatic and aromatic C-H bonds of aromatic 2,5-diketopiperazines. Chembiochem. 2019, 20: 1068-1077.
- Jiang G, Zuo R, Zhang Y, Powell MM, Zhang P, Hylton SM, Loria R, Ding Y. One-pot biocombinatorial synthesis of herbicidal thaxtomins. ACS Catal. 2018, 8: 10761-10768.
- Zhang Y, Chen M, Bruner SD, Ding Y. Heterologous production of microbial ribosomally synthesized and post-translationally modified peptides. Front Microbiol. 2018, 9:1801. doi: 10.3389/fmicb.2018.01801.
- Hanson A, Amthor J, Sun J, Niehaus T, Gregory J, Bruner SD, Ding Y. Redesigning thiamin synthesis: Prospects and potential payoffs, Plant Sci. 2018, 273:92-99.
- Jiang G, Zhang Y, Powell MM, Zhang P, Zuo R, Zhang Y, Kallifidas D, Tieu AM, Luesch H, Loria R*, Ding Y*. High-yield production of herbicidal thaxtomins and analogs in a nonpathogenic Streptomyces strain. Appl. Environ. Microbiol. 2018, 84. pii: AEM.00164-18. doi: 10.1128/AEM.00164-18. (*: co-corresponding author)
- Kallifidas D, Jiang G, Ding Y, Luesch H. Rational engineering of Streptomyces albus J1074 for the overexpression of secondary metabolite gene clusters, Microb. Cell Fact. 2018, 17:25, doi: 10.1186/s12934-018-0874-2.
- Zhang Y, Li K, Yang G, McBride JL, Bruner SD, Ding Y. A distributive peptide cyclase processes multiple microviridin core peptides within a single polypeptide substrate, Nat. Commun. 2018, 9: 1780, doi:10.1038/s41467-018-04154-3
- Yang G, Cozad M, Holland D, Zhang Y, Luesch H, Ding Y. Photosynthetic production of sunscreen shinorine using an engineered cyanobacterium, ACS Syn. Biol. 2018, 7: 664-671.
- Zhang Y, Jiang G, Ding Y*, Loria R*. Genetic background affects pathogenicity island function and pathogen emergence in Streptomyces, Mol. Plant Pathol. 2018, 19: 1733-1741. (*: co-corresponding author)
- Yang G, Zhang Y, Lee NK, Cozad MA, Kearney SE, Luesch H, Ding Y. Cyanobacterial Sfp-type phosphopantetheinyl transferases functionalize carrier proteins of diverse biosynthetic pathways, Sci. Rep. 2017, 7: 11888. doi: 10.1038/s41598-017-12244-3.
- Zhang P, Li K, Yang G, Xia C, Polston JE, Li G, Li S, Lin Z, Yang LJ, Bruner SD, Ding Y. Cytotoxic protein from the mushroom Coprinus comatus possesses a unique mode for glycan binding and specificity, Proc. Natl. Acad. Sci. U. S. A. 2017, 114: 8980-8985.
- Basak A, Abouelhassan Y, Zuo R, Yousaf H, Ding Y, Huigens RW. Antimicrobial peptide-inspired NH125 analogues: bacterial and fungal biofilm-eradicating agents and rapid killers of MRSA persisters, Org. Biomol. Chem. 2017, 15: 5503-5512.
- Zuo R, Zhang Y, Jiang C, Hackett JC, Loria R, Bruner SD, Ding Y. Engineered P450 biocatalysts show improved activity and regio-promiscuity in aromatic nitration, Sci. Rep. 2017, 7: 842. doi: 10.1038/s41598-017-00897-z.
- Li K, Condurso H, Li G, Ding Y, Bruner, SD. Precursor protein-directed peptide macrocyclization in ribosomal peptide biosynthetic pathways, Nat. Chem. Biol. 2016, 12: 973-979.
- Zhang Y, Bignell DR, Zuo R, Fan Q, Huguet-Tapia JC, Ding Y, Loria R. Promiscuous pathogenicity islands and phylogeny of pathogenic Streptomyces spp. Mol. Plant. Microbe Interact. 2016, 29: 640-650.
- Zhang Y, Xie C, Wang H, Foss RM, Clare M, George EV, Li S, Katz A, Cheng H, Ding Y, Tang D, Reeves WH, Yang LJ. Irisin exerts dual effects on browning and adipogenesis of human white adipocytes. Am. J. Physiol. Endocrinol. Metab. 2016, 311: E530-541.
- Tu D, Cheng X, Gao Y, Yang P, Ding Y, Jiang C. Palladium-catalysed direct C-2 methylation of indoles. Org. Biomol. Chem. 2016, 14: 7443-7446.
- Zuo R, Garrison AT, Basak A, Zhang P, Huigens RW*, Ding Y*. In vitro antifungal and antibiofilm activities of halogenated quinoline analogues against Candida albicans and Cryptococcus neoformans. Int. J. Antimicrob. Agents. 2016, 48: 208-211. (*: co-corresponding author)
- Le J, Gao Y, Ding Y*, Jiang C*. Cu-mediated C2-dehydrogenative homocoupling of indoles via C-H activation assisted by a removable N-pyrimidyl group. Tetrahedron Lett. 2016, 57, 1728-1731 (*: co-corresponding author)
- Zuo R, Zhang Y, Huguet-Tapia JC, Mehta M, Dedic E, Bruner SD, Loria R, Ding Y. An artificial self-sufficient cytochrome P450 directly nitrates fluorinated tryptophan analogs with different regio-selectivity. Biotechnol. J. 2016 Jan 7. doi: 10.1002/biot.201500416.
- Zhang Y, Loria R, Ding Y. Applications of natural products from soil microbes. Soil Ecosystem Servicesdoi:10.2136/2015.soilecosystemsservices.2015.0023
- Xie C, Zhang Y, Tran TD, Wang H, Li S, George EV, Zhuang H, Zhang P, Kandel A, Lai Y, Tang D, Reeves WH, Cheng H, Ding Y*, Yang LJ*. Irisin controls growth, intracellular Ca2+ signals, and mitochondrial thermogenesis in cardiomyoblasts. Plos One 2015, 10: e0136816. (*: co-corresponding author)
- Yang G, Ding Y. Recent advances in biocatalyst discovery, development and their applications. Bioorg. Med. Chem. 2014, 22: 5604-5612.
Selected Publications Prior to Independent Career
- Ding Y, Rath CM, Bolduc KL, Håkansson K, Sherman DH. Chemoenzymatic synthesis of cryptophycin anticancer agents by an ester bond-forming non-ribosomal peptide synthetase module. J. Am. Chem. Soc. 2011, 133:14492-14495.
- Ding Y, Wet JR, Cavalcoli J, Li S, Greshock TJ, Miller KA, Finefield JM, Sunderhaus JD, McAfoos TJ, Tsukamoto S, Williams RM, Sherman DH Genome-based characterization of two prenylation steps in the assembly of the stephacidin and notoamide anticancer agents in a marine Aspergillus J. Am. Chem. Soc. 2010, 132: 12733-12740.
- Ding Y, Gruschow S, Greshock TJ, Finefield J, Sherman DH, Williams RW. Detection of VM55599 and Pre-paraherquamide from Aspergillus japonicus and Penicillium fellutanum: Biosynthetic Implications. J. Nat. Prod.2008, 71: 1574-1578
- Ding Y, Williams RM, Sherman DH. Isolation and characterization of a 4-dimethylallyltryptophan synthase from Malbranchea aurantiaca. J. Biol. Chem. 2008, 283: 16068-16076
- Ding Y, Seufert WH, Beck ZQ, Sherman DH. Analysis of the cryptophycin P450 epoxidase reveals substrate tolerance and cooperativity. J. Am. Chem. Soc. 2008, 130: 5492-5498